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Technical data


Reaction kinetics with spectrophotometry

Item No. P3070601

Principle

The organic dye crystal violet is decolorised in an alkaline medium. This reaction takes place according to a velocity-time law, hence it is a reaction of the first order.
Using the integrated software, the change in concentration of the dye can be traced by measuring the extinction at the wavelength of the absorption maximum. The computer program plots the kinetic data in graphical form.

Benefits

  • Modern, easy-to-use photospectrometer
  • Simple evaluation by integrated software

Tasks

The order of reaction during decolorisation of crystal violet is to be determined.

Learning objectives

  • Reaction kinetics
  • First order reaction
  • Photometry
  • Reaction rate

Necessary accessories

  • Precision balance 620g/0.001g

Scope of supply

Fibre-optics spectrophotometer 35620-00 1
Volumetric flasks, Boro, various sizes 36548-00 2
Bulb pipettes, various sizes 36578-00 2
Pipettor 36592-00 1
Beakers, Boro, high form, various sizes 46026-00 3
Microspoon, steel 33393-00 1
Powder funnels, plastic, various sizes 36893-00 1
Wash bottle, plastic, 500 ml 33931-00 1
Sodium hydroxide, pellets, 500 g 30157-50 1
Water, distilled 5 l 31246-81 1
Crystal violet f.bacteriology,25g 31488-04 1
Data cable USB, plug type A/B, 1.8 m 14608-00 1
Macro-cuvettes, PS, 4ml,100 pcs 35663-10 1

PHYWE Systeme GmbH & Co. KG
Robert-Bosch-Breite 10 – 37079 Göttingen – Germany
www.phywe.com