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Technical data


Electrophoresis of Lambda DNA

Item No. P8110600

Principle

The most important work methods of molecular genetics are taught in this experiment. With the aid of agarose gel electrophoresis migration velocity and the cleavage pattern of lambda DNA can be investigated.

Benefits

  • Complete equipment set which also contains all required consumables
  • Consumables can be purchased as a replacement kit
  • Ready to use: DNA fragment samples are already predigested
  • Easy to use: detailed experiment guide
  • DNA samples can can be stored for a longer period at room temperature because they are lyophilized

Tasks

  • Cast the agarose gel
  • Prepare samples for electrophoresis
  • Perform electrophoresis
  • Stain the samples and analyse the banding pattern

Learning objectives

  • Lambda DNA
  • Electrophoresis
  • Restriction enzymes
  • Restriction digestion
  • Banding pattern

Scope of supply

Microliterpipette 2-20 µl, autoclavable 47141-10 1
blueGel gel - electrophoresis unit with integrated illuminator 35016-99 1
Pipette tips, 2-200 µl, 1000 pcs 47148-01 1
Spoon spatula, nickel-plated, 180 mm 33392-00 1
Protecting glasses, clear glass 39316-00 1
Erlenmeyer flasks, Boro, wide neck, various sizes 46151-00 1
Graduated cylinders, Boro, various sizes 36629-00 1
Storage bottle, clear glass, screw cap, 250 ml 34155-00 1
Graduated cylinders, Boro, various sizes 36630-00 1
Water, distilled 5 l 31246-81 1
500 ml, 10x TBE-buffer for gel-electrophoresis KLA-530-223 1
Rubber gloves, size M (8), one pair 39323-00 1
Graduated pipettes, various sizes 36602-00 1
Kit: Agarose gel electrophoresis of Lambda DNA KLA-530-110 1
Pipettor 36592-00 1
SeeGreen™ Agarose Tabs™, 3-in-1 agarose tablets 35018-71 1

PHYWE Systeme GmbH & Co. KG
Robert-Bosch-Breite 10 – 37079 Göttingen – Germany
www.phywe.com